Amgen Inc. v. F. Hoffmann-LaRoche LTD et al

Filing 323

Response by Amgen Inc. to #311 Brief (Defendants' Claim Construction Brief). (Attachments: #1 Exhibit 1-Declaration of Harvey Lodish in Support of Defendants' Claim Construction Brief#2 Exhibit A-to Lodish Declaration#3 Exhibit B-Part 1 of 2 to Lodish Declaration#4 Exhibit B-Part 2 of 2 to Lodish Declaration#5 Exhibit C-to Lodish Declaration#6 Exhibit D- to Lodish Declaration#7 Exhibit E - Part 1 of 2 - to Lodish Declaration#8 Exhibit E-Part 2 of 2 - to Lodish Declaration#9 Exhibit 2 - Declaration of Vladimir Torchilin, Ph.D, D.Sc.#10 Exhibit A-to Torchilin Declaration#11 Exhibit B-Part 1 of 5 - to Torchilin Declaration#12 Exhibit B- Part 2 of 5 to Torchilin Declaration#13 Exhibit B-Part 3 of 5 to Torchilin Declaration#14 Exhibit B-Part 4 of 5 to Torchilin Declaration#15 Exhibit B-Part 5 of 5 to Torchilin Declaration#16 Exhibit C- Part 1 of 2 to Torchilin Declaration#17 Exhibit C-Part 2 of 2 to Torchilin Declaration#18 Exhibit D-Part 1 of 2 to Torchilin Declaration#19 Exhibit D-Part 2 of 2 to Torchilin Declaration#20 Exhibit E - Part 1 of 3 to Torchilin Declaration#21 Exhibit E-Part 2 of 3 to Torchilin Declaration#22 Exhibit E - Part 3 of 3 to Torchilin Declaration#23 Exhibit F to Torchilin Declaration#24 Exhibit 3)(Gottfried, Michael)

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Amgen Inc. v. F. Hoffmann-LaRoche LTD et al Doc. 323 Att. 5 Case 1:05-cv-12237-WGY Document 323-6 Filed 03/19/2007 Page 1 of 9 LODISH DECLARATION EXHIBIT C Dockets.Justia.com Case 1:05-cv-12237-WGY Document 323-6 Filed 03/19/2007 Page 2 of 9 Lin Example 10 Vector Construction huma n mous e virus EPO upstream DNA EPO structural gene DHFR gene DNA viral promoter DNA Recombinant DNA techniques DHFR gene DNA vira l promote r DNA EPO s truc tura l ge ne EPO ups tre a m DNA E P O E x pre s s ion C ons truc t Lin 1 Case 1:05-cv-12237-WGY Document 323-6 Filed 03/19/2007 Page 3 of 9 Lin Example 10 Transfection T ra ns fe c tion EPO Expression Construct Chromosome DNA Lin 2 Case 1:05-cv-12237-WGY Document 323-6 Filed 03/19/2007 Page 4 of 9 Lin Example 10 Transfection / Recombination / Integration Transfection EPO expression construct Chromosome Recombination EPO expression construct Chromosome Integration Recombinant Chromosome Lin 3 Case 1:05-cv-12237-WGY Document 323-6 Filed 03/19/2007 Page 5 of 9 Lin Example 10 L Recombinant Chromosome R e c ombina nt C hromos ome DNA R a ndom I nte gra tion in 4 Case 1:05-cv-12237-WGY Document 323-6 Filed 03/19/2007 Page 6 of 9 Lin Example 10 EPO Protein Expression EPO upstream DNA ATG viral promoter EPO structural gene 1 exon 2 3 4 intron 5 re c ombina nt c hromos ome 1. Transcription AUG 1 2 3 4 5 prima ry R N A tra ns c ript 2. Splice Donor-Acceptor Alignment AUG 1 2 3 4 5 splice donor splice acceptor tra ns c ript s plic ing 3. Splicing AUG 12345 mR N A 4. Translation le a de r pe ptide polype ptide 5. Post-translational Modification EPO glyc oprote in le a de r pe ptide c le a ve d c te rmina l a rginine c le a ve d Lin 5 Case 1:05-cv-12237-WGY Document 323-6 Filed 03/19/2007 Page 7 of 9 Lin Example 10 DNA Amplification MTX Vast majority of the cells die, but cells with amplified DNA survive Lin 6 Case 1:05-cv-12237-WGY Document 323-6 Filed 03/19/2007 Page 8 of 9 Lin Example 10 L Re-Amplification Cultured cells containing amplified DNA Additional cycles of MTX selection Cells with further amplified DNA survive in 7 Case 1:05-cv-12237-WGY Document 323-6 Filed 03/19/2007 Page 9 of 9 Lin Example 10 Process for EPO Production Cells with amplified EPO DNA Production of EPO protein Purification of EPO protein Pharmaceutical EPO Lin 8

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